منابع مشابه
Chimeric cardiac troponin I antibodies
most assay manufacturers take additional measures during the assay development. The most frequent procedure involves suppressing the non-specific binding with blocking reagents, which vary from normal mouse IgG to more refined formulations. Whilst in most cases these measures are adequate, they do result in additional costs in terms of both material and labor. The effect of HAMA can also be sup...
متن کاملDegradation of cardiac troponin I in serum complicates comparisons of cardiac troponin I assays.
BACKGROUND Up to a 20-fold variation in serum cardiac troponin I (cTnI) concentration may be observed for a given patient sample with different analytical methods. Because more limited variation is seen for control materials and for purified cTnI, we explored the possibility that cTnI was present in altered forms in serum. METHODS We used four recombinantly engineered cTnI fragments to study ...
متن کاملHydrodynamic properties of bovine cardiac troponin-I and troponin-T.
Bovine cardiac troponin-I (TN-I) and troponin-T (TN-T) have been examined in solution using ultracentrifugation, gel filtration, and viscosity. A new method of purifying TN-T, employing hydroxylapatite chromatography in 6 M urea, is reported. Cardiac TN-T (Mr = 36,000) undergoes a reversible, concentration-dependent association in nondenaturing buffers, probably to a tetramer. The Stokes radius...
متن کاملCardiac Troponin I elevation after epileptic seizure
BACKGROUND Cardiac troponin-I (cTNI) is highly specific biomarker to prove myocardial damage, e.g. in acute coronary syndrome (ACS). However, it occurs in other conditions as well. We therefore analysed cTNI increase in patients after generalized convulsive seizure. METHODS Consecutive patients admitted with acute generalized convulsive seizure were included in case of cTNI measurement on adm...
متن کاملEvaluation of cardiac troponin I stability
ve markers of cardiovascular mortality, notably in patients undergoing vascular surgery3, non-cardiac surgery4, traumatic cardiac injury5 or in pregnant women suffering from pre-eclampsia6. Cardiac troponins are thus extensively prescribed in various clinical situations, and their analysis must be carried in appropriate conditions to reach the highest possible accuracy. In France, to reach opti...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 2007
ISSN: 0009-7330,1524-4571
DOI: 10.1161/circresaha.107.165258